Tony Hunter, American Cancer Society Professor, Molecular and Cell Biology Laboratory, Renato Dulbecco Chair, Salk Institute
Resumen de la ponencia
I will discuss our recent studies on protein histidine phosphorylation and its function as a reversible posttranslational modification. I will describe how we developed monoclonal antibodies specific for the 1-pHis and 3-pHis isoforms, and have used them to define the size of the cellular phosphohistidine (pHis) proteome and to identify individual sites of histidine phosphorylation, and then use this information to investigate the role that histidine phosphorylation plays in regulating different cellular processes. I will focus on possible roles for histidine phosphorylation in several different cancers, including pancreatic cancer, and link this to work that we have been doing to decipher the role of secreted factors in the intercellular communication between different stromal cell types and tumor cells during the development and progression of pancreatic cancer.